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1996-03-04
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Document 0780
DOCN M9640780
TI CD4 enumeration in HIV infected individuals.
DT 9604
AU Lopez A; Ciudad J; Cordero M; Orfao A; Servicio General de Citometria,
University of Salamanca, Spain.
SO Eur J Histochem. 1994;38 Suppl 1:35-40. Unique Identifier : AIDSLINE
MED/96120977
AB In the present study, two alternative methods for the enumeration of PB
CD4+ lymphocytes are compared with conventional flow cytometry (FCM):
the TRAX CD4 tm enzyme immunoassay test kit and a new method using an
internal biological standard (liophilized lymphocytes) for the direct
enumeration of total PB CD4+ T-cells. Both methods showed an overall
high degree of correlation with conventional FCM. However, the TRAxCD4
tm enzyme immunoassay showed a significant overestimation of PB CD4+
T-cells that was not only due to monocyte-derived CD4 molecules and the
use of liophilized lymphocytes as an internal biological standard showed
a low level of correlation for patients displaying low CD4 counts.
DE Cell Count/METHODS Comparative Study CD4 Lymphocyte Count/*METHODS
Flow Cytometry Human HIV Infections/*BLOOD Immunoenzyme Techniques
JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).